A2.3 Viruses
New to Syllabus
Higher Level
Introduction to Viruses
A virus is a microscopic infectious agent that is fundamentally different from living cells. It is not considered a living organism because it lacks the cellular machinery required for metabolism, energy production, and independent reproduction. Instead, viruses are obligate intracellular parasites, meaning they can only reproduce by infecting a host cell and hijacking its molecular machinery.
Higher Level
Higher Level
Structural features of Viruses
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Very small size (much smaller than bacteria).
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No growth or metabolic processes on their own.
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Nucleic acid: either DNA or RNA (not both).
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Capsid: protein coat enclosing the genetic material.
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No cytoplasm, organelles, or viral enzymes (until inside host).

Higher Level
Higher Level
Higher Level
Diversity Among Viruses
1. Genetic Material
May contain DNA or RNA, and it can be single or double stranded.
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Further variation in how RNA is processed:
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Positive-sense RNA: acts directly as mRNA.
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Negative-sense RNA: must be transcribed to mRNA.
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Retroviruses: RNA → DNA via reverse transcriptase (e.g. HIV).
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2. Enveloped vs. Non-Enveloped
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Enveloped viruses: have a lipid membrane derived from the host.
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Non-enveloped viruses: lack a membrane, typically more stable.

Higher Level
Examples

Higher Level
Lytic vs Lysogenic Cycles
Lytic Cycle: Occurs mostly in plants and animals. Leads to host cell lysis.
Steps:
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Attachment
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DNA Entry
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DNA Replication
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Transcription
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Protein Synthesis (self-assembly)
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Lysis (break host membrane)
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Spread to nearby cells
Lysogenic Cycle: Virus integrates DNA into host genome and remains dormant & can later activate and enter lytic cycle.
Steps:
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Attachment
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DNA Entry
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Integration via integrase enzyme
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(→ Viral DNA inserted at a specific site in host chromosome)
Temperate viruses: do not lyse host immediately (e.g., phage λ).


Higher Level
Higher Level
Evidence for origin of viruses
Progressive Hypothesis: Viruses evolved from mobile genetic elements (e.g., plasmids).
Regressive Hypothesis: Viruses were once free-living cells that lost complexity over time.
Higher Level
Higher Level
Rapid Evolution of Viruses
Viruses mutate rapidly due to:
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Short generation times
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High mutation rates
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Strong natural selection pressure
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